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KMID : 0613820080180060878
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Journal of Life Science
2008 Volume.18 No. 6 p.878 ~ p.883
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Improvement of the Phosphate Solubilization Microorganism by the Introduction of Glucose Dehydrogenase Gene into Aeromonas hydrophila DA33
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Park In-Hye
Song Ok-Ryul
Lee Yong-Seok
Kang Ui-Gum
Choi Si-Lim
Choe Yong-Lark
Choi Yong-Lark
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Abstract
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DA33 was isolated from cultivated soils as a bacteria having high abilities to solubilize inorganic phosphate. Glucose dehydrogenase gene (gdh) was cloned from Escherichia coli. The recombinant plasmid, pGHS containing glucose dehydrogenase gene was introduced into A. hydrophila DA33 in order to improve the activity of phosphate-solubilizing. The transformant harboring the gdh gene, A. hydrophila pGHS/DA33 increased enzyme activity. The strain also increased the gluconic acid generation that was effective for phosphate solubilization. It was possible that the strain containing pGHS produced higher solubilized phosphate with tri-calcium phosphate as the unique (P) source, in comparison with that of wild type without plasmid. These results suggest that the strain, A. hydrophila pGHS/DA33 is expected as effective biofertilizer for phosphate solubilization.
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KEYWORD
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Aeromonashydrophila, gluconicacid, glucosedehydrogenasegene, phosphate-solubilizingmicroorganism
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